Product Details
  • 产品名称:ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM

  • 产品型号:
  • 产品厂商:Elctroporation
  • 产品价格:0
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简单介绍:
The ECM 2001 is a multipurpose electro cell manipulation pulse generator capable of performing both electrofusion for hybridoma production, hybrid cell formation and nuclear transfer applications in addition to electroporation of cells in suspension and in vivo applications. This versatile platform combines microprocessor-controlled precision with a wide range of possible parameters for a wide ran
详情介绍:

The ECM 2001 multi-purpose electro cell manipulation generator produces a proprietary AC waveform for electrophoretic alignment of cells and a square DC waveform for cell fusion.

ECM 2001 GENERATOR

The ECM 2001 is a multipurpose electro cell manipulation pulse generator capable of performing both electrofusion for hybridoma production, hybrid cell formation and nuclear transfer applications in addition to electroporation of cells in suspension and in vivo applications. This versatile platform combines microprocessor-controlled precision with a wide range of possible parameters for a wide range of applications when combined with standard cuvettes, high throughput plates, specialty electrodes and other available accessories.

The ECM 2001 is intended For Research Use Only.  Not for use in diagnostic, pre-clinical, or clinical procedures.

FEATURES

  • Wide range of voltages from 5 to 3000 V
  • Finer voltage discrimination
  • Pulse durations from 10 µsec to 10 sec
  • Arc quenching
  • Digital display of actual voltage and pulse length delivered

FUSION

The ECM 2001 is capable of producing a proprietary AC waveform for benign dielectrophoretic alignment of cells, and a square DC waveform for cell fusion. Electrofusion is carried out by applying both the AC and DC pulse waveforms. Microsecond switch-over time from AC to DC allows for highly efficient fusion. After fusion, the AC pulse reapplication maintains compression of the cells following the DC pulse for the rounding off process; resulting in a higher number of hybrids. The 1 MHz AC pulse aligns cells together in real time, saving time (seconds vs. hours) in comparison to PEG methods. Blastomeres and oocytes can also be aligned to the correct position for better fusion accuracy during nuclear transfer and embryo manipulation.

ELECTROPORATION

The ECM 2001 EP System is designed for transfection of eukaryotic cells in suspension using electroporation cuvettes. 

Utilizing the ECM 2001 for standard mammalian cell transfections is simple; the AC feature is turned off and the DC mode is set as a square wave electroporation device. The range of voltages and pulse lengths that can be programmed, coupled with the ability to carry out up to 9 pulses per experiment, make this an extremely versatile system for any lab. 

ADHERENT CELL TRANSFECTION

Electroport adherent cells directly into the dish used for cell growth. The ECM 2001 coupled with the Petri Pulser electrode or the Petri Dish electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in. The Petri Pulser is ideal for 6-well plates and the Petri Dish Electrode is ideal for 100 mm petri dishes.

HYBRIDOMA PRODUCTION

For use in hybridoma production applications, the ECM 2001 generator, 3.2 mm gap or 10 mm gap Microslides, and connection cables are required. This system does not require proprietary fusion medium, but can be used with the Cytofusion Medium C for convenience.

Electrofusion is achieved by the generation of a proprietary 1 MHz AC waveform for benign dielectrophoretic cell alignment, microsecond switchover from AC to DC creates efficient fusion results. After fusion, the AC pulse reapplication maintains compression of the cells following the DC pulse resulting in a higher number of hybrids.

INCLUDED ITEMS

Item # Product Included Items
45-0010 ECM 2001 Cell Fusion System Complete (N. America) ECM 2001 Generator, Microslides (0.5 mm Gap, 3.2 mm Gap), Meander Fusion Chamber, Flat Electrode / Divergent Field, Electrode Adapter, Connection Cable, Safety Stand 630B, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660
45-0010INT ECM 2001 Cell Fusion System Complete ECM 2001 Generator, Microslides (0.5 mm Gap, 3.2 mm Gap), Meander Fusion Chamber, Flat Electrode / Divergent Field, Electrode Adapter, Connection Cable, Safety Stand 630B, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660
45-0011 ECM 2001 Electroporation System Complete, for Cuvettes (N. America) ECM 2001 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660 
45-0011INT ECM 2001 Electroporation System Complete, for Cuvettes  ECM 2001 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660 
45-0012 ECM 2001 Embryo Manipulation System (N. America) ECM 2001 Generator, Microslides, round wire (0.5 mm gap,1.0 mm gap), rectangular wire (3.2 mm gap), Micrograbber adapter cables
45-0012INT ECM 2001 Embryo Manipulation System ECM 2001 Generator, Microslides, round wire (0.5 mm gap,1.0 mm gap), rectangular wire (3.2 mm gap), Micrograbber adapter cables
45-0018 ECM-2001 Hybridoma Production System (N. America) ECM 2001 Generator, Microslides, rectangular wire (3.2 mm gap, 10 mm gap), Adapter Set, Micrograbber adapter cables, BTX Cytofusion Medium C, 500 ml, Safety Stand for Flatpack
45-0018INT ECM-2001 Hybridoma Production System ECM 2001 Generator, Microslides, rectangular wire (3.2 mm gap, 10 mm gap), Adapter Set, Micrograbber adapter cables, BTX Cytofusion Medium C, 500 ml, Safety Stand for Flatpack
45-0080 ECM 2001 Generator Only (N. America) ECM 2001 Generator only
45-0080INT ECM 2001 Generator Only ECM 2001 Generator only
45-0013 ECM 2001 System Complete, for Cuvettes with Monitoring  (N. America) ECM 2001 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660, ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope and Cables
45-0013INT ECM 2001 System Complete, for Cuvettes with Monitoring  ECM 2001 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660, ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope and Cables
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